Metabolic profiling tools using full metabolic labeling have been developed in order to make the separation between control and experimental cell population simpler using 95% 13C- and 5% 13C- based media. By following complete labeling, 95% 13C- and 5% 13C cell populations may be pooled before examination. This is because they show unique mass spectral patterns. This has in it the benefits of eradicating sample-to-sample variance. Their unique patterns also make the identification and quantitation of real metabolite peaks easy as unlabeled non-biological peaks can be removed from the dataset. In the following passages let’s learn more things about cellculture media to C13 label metabolites.
Some significant factors about cell culture
Cell culture is said to be one of the vital techniques of life sciences. Cell culture is a regular term that is used for describing the replacement of the organs, cells or tissues from a plant or animal and their subsequent placing into an artificial environment favourable to their survival and growth.
The basic environmental fundamentals for the most excellent progression of cells are as portrayed below:
- Substrate for the attachment of cells
- Suitable temperature
- Favourable medium for growth
- An incubator for maintaining appropriate growth medium for artificial cultivation.
The growth medium or culture medium is actually a gel or liquid base projected to endorse the growth of small plants, cells or microorganisms. Usually, cell culture media includes an appropriate resource of energy and compounds for regulating the cell cycle.
Know about the importance of metabolic profiling in cell culture media
conventionally in-vitro metabolite stable isotope labeling has been carried out by incorporate 99% C13 through feeding cells with isotopically labeled media, like making use of a medium with a sole carbon source of C13-glucose and quantifying by evaluating the profiles of labeled metabolites vs. unlabeled metabolites from a control population making use of analytical processes like mass spectrometry to identify differentiating metabolites or to find out new metabolites. though, acquiring precise, identification, and totally reproducible quantitation, making use of these systems can be really very challenging.
What are the advantages of 95% and 5% 13C labelling?
Eliminating false data – 95% and 5% 13C gives rise to a distinguishing isotopic pattern with no trouble, differentiated from natural abundance non-biological artefacts and noise.
Mathematically calculable to enable computational analysis
Effectively removes variances – labelled and unlabeled species are subject to the same biases, thus growing comparability of different measurements.
Allows recognition – the number of carbons for each and every metabolite can be determined; carbon number and mass together really confine the number of probable molecular formulae.
Makes detections of low intensity characteristics easy – at natural profusion more than one isotopic peak is frequently not noticeable or can be excluded as noise.
Decreases cost of examination – 95% and 5% 13C samples can surely be mixed and analyzed together, reducing sample number by half.
Interested to explore more?
Contact with IROA Technologies, a well-reputed metabolomics company. They can offer high quality metabolic profiling tools including cell culture media to C13 label metabolites labelling based on 95% and 5% C13. Visit iroatech.com For further information.