Large clinically-relevant metabolic profiling requires accurate identification, reproducible quantitation, and performance criteria for instrumentation. For this, you must use advanced metabolic profiling tools.
Today, we are going to talk about the IROA quantitation kits that you can use for MS-based metabolic profiling studies. These kits contain the reagents that are necessary to label, quantitate, and identify biochemical compounds successfully. For example, the IROA PHENO-95-300 Kit contains 13C yeast extract. You can create your own internal standards that are required for accurate and reproducible metabolic profiling.
However, IROA (Isotopic Ratio Outlier Analysis) is actually a simple and direct metabolic profiling technique that involves embedding unique signatures (tags), specifically using 95% and 5% 13C abundance, into biochemical metabolites for relative quantitation by mass spectrometry and software algorithms for finding, identifying, and quantitating these tags.
There are many advantages of using metabolite C13-labeled internal standards. First, it enhances the data quality through the removal of noise, quality assurance, compound identification & quantitation. C13-labeled internal standards also provide the means for data standardization.
Now, let’s get started with IROA Quantitation kits.
IROA Quantitation Kits for Metabolic Profiling
Here are the IROA quantitation kits that you can use for Yeast & fungi, bacterial, and mammalian profiling:
1. IROA®100 for Yeast and Fungal Metabolic Profiling
You can create yeast internal standards, identify & quantitate yeast metabolites using this kit. The kit is supplied with YNB media and energy sources. It provides all the reagents that are necessary for labeling, quantitating, and identifying biochemical compounds in many yeast and fungal cell populations successfully when combined with mass spectrometry and sample preparation. You can quantitate metabolic differences between the two groups of cell populations (a control group and an experimental group) using the IROA 100 quantitation kit.
The kit components include IROA® 12C Biochemical Labeling mix (50 mL YNB containing 1.0 g of 5% 13C labeled glucose), IROA® 13C Biochemical Labeling mix (50 mL YNB containing 1.0 g of 95% 13C labeled glucose), IROA ClusterFinder™, and Software Product User Manual.
2. IROA®200 for Bacterial Metabolic Profiling
You can use this kit to create bacterial internal standards, identify & quantitate bacterial metabolites. The kit is supplied with M9 Minimal Media and energy sources and can be used for labeling, quantitating, and identifying biochemical compounds in many bacterial cell populations when combined with mass spectrometry and sample preparation.
The kit components include IROA® 12C Biochemical Labeling Mix, IROA® 13C Biochemical Labeling Mix, IROA® 12C Amino Acid Labeling Mix, IROA® 13C Amino Acid Labeling mix, 2 DynaGuard Filters, IROA ClusterFinder™ software, and Product User Manual.
3. IROA®300 for Mammalian Metabolic Profiling
It is supplied as 2 kits: IROA® Phenotypic Quantitation Kit and IROA® Fluxomic Quantitation Kit. The IROA® Phenotypic Quantitation Kit is designed for situations where it’s not possible to label experimental samples in culture systems such as biopsies or large-scale fermentation. The Fluxomic Quantitation Kit provides the necessary reagents to fully label mammalian cells at 5% U-13C.
Know more about IROA Metabolic Profiling Tools
You can visit the website – iroatech.com to find detailed information about the yeast, bacterial, and mammalian profiling tools. You will also find other details related to metabolic profiling and 13C yeast extract on the website. Feel free to get in touch with ‘IROA Technologies LLC’ to know more.